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Competency Testing in Aseptic Manipulation

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Video 1 – Introduction

Competency Testing in Aseptic Manipulation: An Introduction

1 Quiz
- Quiz for Competency Testing in Aseptic Manipulation: An Introduction
Video 2 – Initial Garbing for Entering the Cleanroom

Entering the cleanroom – Initial Cleanroom Garbing

1 Quiz
- Quiz for Entering the cleanroom – Initial Cleanroom Garbing
Video 3 – Hand Hygiene & Final Garbing

Hand Hygiene and Final Garbing

1 Quiz
- Quiz for Hand Hygiene and Final Garbing
Video 4 – Donning Sterile Gloves & GFTT

Donning Sterile Gloves & Glove Fingertip Thumb Sampling

1 Quiz
- Quiz for Donning Sterile Gloves & Glove Fingertip Thumb Sampling
Video 5 – Media Fills / Aseptic Process Simulations

Media Fills – Aseptic Process Simulations

1 Quiz
- Quiz for Media Fills – Aseptic Process Simulations
Video 6 – Surface Sampling

Surface Sampling

1 Quiz
- Quiz for Surface Sampling

Quiz 4 of 6

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Quiz for Donning Sterile Gloves & Glove Fingertip Thumb Sampling

Seth DePasquale, R.Ph., BCSCP September 4, 2025

Competency Testing in Aseptic Manipulation
Donning Sterile Gloves & Glove Fingertip Thumb Sampling
Quiz for Donning Sterile Gloves & Glove Fingertip Thumb Sampling

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Question 1 of 10

1. Question

Why are sterile gloves critical in aseptic processing?

They protect the technician from chemical exposure
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They act as a primary barrier to maintain aseptic integrity
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They prevent glove powder contamination
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They replace the need for proper garbing
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Question 2 of 10

2. Question

When donning sterile gloves, which surface can be safely touched with bare hands?

The cuff
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The inside fold of the glove
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The outside fingertip area
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None of the glove surfaces
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Question 3 of 10

3. Question

What should be done after both gloves are donned?

Apply alcohol spray directly to the gloves
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Tuck glove cuffs securely over gown sleeves
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Touch a sterile work surface to verify glove sterility
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Adjust gloves by pulling at the fingertips
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Question 4 of 10

4. Question

What is the purpose of Gloved Fingertip Sampling (GFS) and Media Fill Testing (MFT)?

To test the sterility of disinfectants
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To validate cleanroom environmental control systems
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To confirm aseptic technique, hand hygiene, and garbing practices
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To replace daily environmental monitoring
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Question 5 of 10

5. Question

How many initial GFS tests must a new employee successfully complete before compounding?

One test with 0 CFU
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Two tests with ≤ 3 CFUs
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Three tests with 0 CFU each
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Annual test with 0 CFU
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Question 6 of 10

6. Question

What does the Media Fill Test simulate?

Daily disinfection of surfaces
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Compounding manipulations using nutrient broth
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Air sampling in the PEC
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Verification of incubator function
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Question 7 of 10

7. Question

During gloved fingertip sampling, how should each fingertip be applied to the agar plate?

Press firmly with all fingers at once
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Roll each fingertip and thumb in one smooth motion, one at a time
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Drag the fingers across the surface
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Use the same spot on the plate for all fingers
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Question 8 of 10

8. Question

What is the purpose of including a negative control plate in each sampling session?

To verify agar sterility
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To record baseline CFU counts
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To provide additional incubation plates
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To reduce variability in results
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Question 9 of 10

9. Question

What are the incubation conditions for gloved fingertip plates?

Upright at room temperature for 24 hours
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Inverted at 30–35 °C for 48–72 hours, then 20–25 °C for 5–7 days
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Upright at 37 °C for 14 days
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Inverted at 4 °C for 7 days
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Question 10 of 10

10. Question

What action must be taken if fingertip sampling exceeds compliance thresholds?

Continue compounding but repeat sampling the next day
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Stop compounding, notify supervisor, retrain and retest before resuming
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Ignore the result if only one plate is contaminated
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Disinfect gloves and repeat the test immediately
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Q.I. Medical makes unique products for pharmacists and nurses who handle sterile solutions. Our focus is on devices, test kits & accessories that improve aseptic technique. Applications include environmental monitoring, technique and process validation, microbial and endotoxin contamination testing, filtration, and needleless dispensing.

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