Quiz for Donning Sterile Gloves & Glove Fingertip Thumb Sampling

Why are sterile gloves critical in aseptic processing?

They protect the technician from chemical exposure

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They act as a primary barrier to maintain aseptic integrity

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They prevent glove powder contamination

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They replace the need for proper garbing

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When donning sterile gloves, which surface can be safely touched with bare hands?

The cuff

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The inside fold of the glove

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The outside fingertip area

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None of the glove surfaces

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What should be done after both gloves are donned?

Apply alcohol spray directly to the gloves

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Tuck glove cuffs securely over gown sleeves

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Touch a sterile work surface to verify glove sterility

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Adjust gloves by pulling at the fingertips

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What is the purpose of Gloved Fingertip Sampling (GFS) and Media Fill Testing (MFT)?

To test the sterility of disinfectants

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To validate cleanroom environmental control systems

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To confirm aseptic technique, hand hygiene, and garbing practices

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To replace daily environmental monitoring

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How many initial GFS tests must a new employee successfully complete before compounding?

One test with 0 CFU

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Two tests with ≤ 3 CFUs

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Three tests with 0 CFU each

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Annual test with 0 CFU

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What does the Media Fill Test simulate?

Daily disinfection of surfaces

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Compounding manipulations using nutrient broth

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Air sampling in the PEC

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Verification of incubator function

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During gloved fingertip sampling, how should each fingertip be applied to the agar plate?

Press firmly with all fingers at once

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Roll each fingertip and thumb in one smooth motion, one at a time

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Drag the fingers across the surface

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Use the same spot on the plate for all fingers

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What is the purpose of including a negative control plate in each sampling session?

To verify agar sterility

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To record baseline CFU counts

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To provide additional incubation plates

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To reduce variability in results

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What are the incubation conditions for gloved fingertip plates?

Upright at room temperature for 24 hours

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Inverted at 30–35 °C for 48–72 hours, then 20–25 °C for 5–7 days

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Upright at 37 °C for 14 days

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Inverted at 4 °C for 7 days

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What action must be taken if fingertip sampling exceeds compliance thresholds?

Continue compounding but repeat sampling the next day

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Stop compounding, notify supervisor, retrain and retest before resuming

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Ignore the result if only one plate is contaminated

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Disinfect gloves and repeat the test immediately

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Competency Testing in Aseptic Manipulation

Video 1 – Introduction

Quiz for Donning Sterile Gloves & Glove Fingertip Thumb Sampling

Seth September 4, 2025

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